Review





Similar Products

one step quantitative reverse transcription pcr rt qpcr kit  (Vazyme Biotech Co)
97
Vazyme Biotech Co one step quantitative reverse transcription pcr rt qpcr kit
One Step Quantitative Reverse Transcription Pcr Rt Qpcr Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+pcr/HiScript+II+One+Step+qRT-PCR+SYBR+Green+Kit/pm41944250-203-9-17
Average 97 stars, based on 1 article reviews
one step quantitative reverse transcription pcr rt qpcr kit - by Bioz Stars, 2026-09
97/100 stars
  Buy from Supplier
lenti x quantitative reverse transcription pcr qrt pcr titration kit  (TaKaRa)
96
TaKaRa lenti x quantitative reverse transcription pcr qrt pcr titration kit
( A ) Schematic of flow cytometric analysis and quantification of immune cells in abdominal aortic tissue from the AAA model after 1 week of AngII infusion. ( B ) Representative flow cytometry plots of digested abdominal aortic tissue 1 week after AngII infusion ( n = 5 mice per group). ( C ) Quantification of immune cell populations in digested abdominal aortic tissue (absolute number per 100 mg). Neut, neutrophils; Ly6C hi Mono, Ly6C hi monocytes; Mac, macrophages; CCR2 + Mac, CCR2 + macrophages. Saline, n = 6; AngII, n = 5; B cells and T cells, n = 5 per group. * P < 0.05 and ** P < 0.01, by Mann-Whitney U test. ( D ) Representative images of CD68, α–smooth muscle actin (α-SMA), CD31/platelet endothelial cell adhesion molecule 1 (PECAM-1), and DAPI immunofluorescence staining of abdominal aortic tissue from aortas of control and 20% Tet2 -KO BM recipient mice after 1 week of AngII infusion. Images are representative of 8 mice per genotype. Scale bars: 100 μm. ( E ) Schematic of RNA-seq analysis of sorted macrophages from abdominal aortas of 100% Tet2 -KO BM recipient mice after 1 week of AngII infusion. ( F and G ) Upregulated and downregulated genes are presented as a heatmap ( F ) and volcano plot ( G ). ( H ) Gene Ontology enrichment analysis of upregulated genes in Tet2 -deficient macrophages. The table lists the predominant genes ranked by q value among significantly enriched pathways. ( I and J ) In vitro differentiation of BM-derived macrophages. ( I and J ) Representative images and quantification of the TRAP + cell-to-macrophage ratio after RANKL stimulation from 8 independent biological replicates per genotype. *** P < 0.001, by Mann-Whitney U test. ( K <t>)</t> <t>qRT-PCR</t> analysis of BM-derived macrophages 6 hours after stimulation with 10 ng/mL LPS ( n = 6 independent biological replicates per genotype). **** P < 0.0001, by Mann-Whitney U test.
Lenti X Quantitative Reverse Transcription Pcr Qrt Pcr Titration Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+pcr/Lenti-X+qRT-PCR+Titration+Kit/pmc13078872-192-6-16
Average 96 stars, based on 1 article reviews
lenti x quantitative reverse transcription pcr qrt pcr titration kit - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
quantitative reverse transcription pcr qrt pcr  (Roche)
99
Roche quantitative reverse transcription pcr qrt pcr
( A ) Schematic of flow cytometric analysis and quantification of immune cells in abdominal aortic tissue from the AAA model after 1 week of AngII infusion. ( B ) Representative flow cytometry plots of digested abdominal aortic tissue 1 week after AngII infusion ( n = 5 mice per group). ( C ) Quantification of immune cell populations in digested abdominal aortic tissue (absolute number per 100 mg). Neut, neutrophils; Ly6C hi Mono, Ly6C hi monocytes; Mac, macrophages; CCR2 + Mac, CCR2 + macrophages. Saline, n = 6; AngII, n = 5; B cells and T cells, n = 5 per group. * P < 0.05 and ** P < 0.01, by Mann-Whitney U test. ( D ) Representative images of CD68, α–smooth muscle actin (α-SMA), CD31/platelet endothelial cell adhesion molecule 1 (PECAM-1), and DAPI immunofluorescence staining of abdominal aortic tissue from aortas of control and 20% Tet2 -KO BM recipient mice after 1 week of AngII infusion. Images are representative of 8 mice per genotype. Scale bars: 100 μm. ( E ) Schematic of RNA-seq analysis of sorted macrophages from abdominal aortas of 100% Tet2 -KO BM recipient mice after 1 week of AngII infusion. ( F and G ) Upregulated and downregulated genes are presented as a heatmap ( F ) and volcano plot ( G ). ( H ) Gene Ontology enrichment analysis of upregulated genes in Tet2 -deficient macrophages. The table lists the predominant genes ranked by q value among significantly enriched pathways. ( I and J ) In vitro differentiation of BM-derived macrophages. ( I and J ) Representative images and quantification of the TRAP + cell-to-macrophage ratio after RANKL stimulation from 8 independent biological replicates per genotype. *** P < 0.001, by Mann-Whitney U test. ( K <t>)</t> <t>qRT-PCR</t> analysis of BM-derived macrophages 6 hours after stimulation with 10 ng/mL LPS ( n = 6 independent biological replicates per genotype). **** P < 0.0001, by Mann-Whitney U test.
Quantitative Reverse Transcription Pcr Qrt Pcr, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+pcr/LightCycler+480+System/pm41977762-272-6-16
Average 99 stars, based on 1 article reviews
quantitative reverse transcription pcr qrt pcr - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
reverse transcription quantitative pcr rt qpcr  (Toyobo)
99
Toyobo reverse transcription quantitative pcr rt qpcr
( A ) Schematic of flow cytometric analysis and quantification of immune cells in abdominal aortic tissue from the AAA model after 1 week of AngII infusion. ( B ) Representative flow cytometry plots of digested abdominal aortic tissue 1 week after AngII infusion ( n = 5 mice per group). ( C ) Quantification of immune cell populations in digested abdominal aortic tissue (absolute number per 100 mg). Neut, neutrophils; Ly6C hi Mono, Ly6C hi monocytes; Mac, macrophages; CCR2 + Mac, CCR2 + macrophages. Saline, n = 6; AngII, n = 5; B cells and T cells, n = 5 per group. * P < 0.05 and ** P < 0.01, by Mann-Whitney U test. ( D ) Representative images of CD68, α–smooth muscle actin (α-SMA), CD31/platelet endothelial cell adhesion molecule 1 (PECAM-1), and DAPI immunofluorescence staining of abdominal aortic tissue from aortas of control and 20% Tet2 -KO BM recipient mice after 1 week of AngII infusion. Images are representative of 8 mice per genotype. Scale bars: 100 μm. ( E ) Schematic of RNA-seq analysis of sorted macrophages from abdominal aortas of 100% Tet2 -KO BM recipient mice after 1 week of AngII infusion. ( F and G ) Upregulated and downregulated genes are presented as a heatmap ( F ) and volcano plot ( G ). ( H ) Gene Ontology enrichment analysis of upregulated genes in Tet2 -deficient macrophages. The table lists the predominant genes ranked by q value among significantly enriched pathways. ( I and J ) In vitro differentiation of BM-derived macrophages. ( I and J ) Representative images and quantification of the TRAP + cell-to-macrophage ratio after RANKL stimulation from 8 independent biological replicates per genotype. *** P < 0.001, by Mann-Whitney U test. ( K <t>)</t> <t>qRT-PCR</t> analysis of BM-derived macrophages 6 hours after stimulation with 10 ng/mL LPS ( n = 6 independent biological replicates per genotype). **** P < 0.0001, by Mann-Whitney U test.
Reverse Transcription Quantitative Pcr Rt Qpcr, supplied by Toyobo, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+pcr/THUNDERBIRD+Probe+qPCR+Mix/pm41595206-108-0-13
Average 99 stars, based on 1 article reviews
reverse transcription quantitative pcr rt qpcr - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
quantitative reverse transcription pcr qrt pcr  (Bio-Rad)
99
Bio-Rad quantitative reverse transcription pcr qrt pcr
( A ) Schematic of flow cytometric analysis and quantification of immune cells in abdominal aortic tissue from the AAA model after 1 week of AngII infusion. ( B ) Representative flow cytometry plots of digested abdominal aortic tissue 1 week after AngII infusion ( n = 5 mice per group). ( C ) Quantification of immune cell populations in digested abdominal aortic tissue (absolute number per 100 mg). Neut, neutrophils; Ly6C hi Mono, Ly6C hi monocytes; Mac, macrophages; CCR2 + Mac, CCR2 + macrophages. Saline, n = 6; AngII, n = 5; B cells and T cells, n = 5 per group. * P < 0.05 and ** P < 0.01, by Mann-Whitney U test. ( D ) Representative images of CD68, α–smooth muscle actin (α-SMA), CD31/platelet endothelial cell adhesion molecule 1 (PECAM-1), and DAPI immunofluorescence staining of abdominal aortic tissue from aortas of control and 20% Tet2 -KO BM recipient mice after 1 week of AngII infusion. Images are representative of 8 mice per genotype. Scale bars: 100 μm. ( E ) Schematic of RNA-seq analysis of sorted macrophages from abdominal aortas of 100% Tet2 -KO BM recipient mice after 1 week of AngII infusion. ( F and G ) Upregulated and downregulated genes are presented as a heatmap ( F ) and volcano plot ( G ). ( H ) Gene Ontology enrichment analysis of upregulated genes in Tet2 -deficient macrophages. The table lists the predominant genes ranked by q value among significantly enriched pathways. ( I and J ) In vitro differentiation of BM-derived macrophages. ( I and J ) Representative images and quantification of the TRAP + cell-to-macrophage ratio after RANKL stimulation from 8 independent biological replicates per genotype. *** P < 0.001, by Mann-Whitney U test. ( K <t>)</t> <t>qRT-PCR</t> analysis of BM-derived macrophages 6 hours after stimulation with 10 ng/mL LPS ( n = 6 independent biological replicates per genotype). **** P < 0.0001, by Mann-Whitney U test.
Quantitative Reverse Transcription Pcr Qrt Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+pcr/CFX96+Touch/pm41898474-323-0-8
Average 99 stars, based on 1 article reviews
quantitative reverse transcription pcr qrt pcr - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
reverse transcription quantitative pcr rt qpcr  (Vazyme Biotech Co)
96
Vazyme Biotech Co reverse transcription quantitative pcr rt qpcr
( A ) Schematic of flow cytometric analysis and quantification of immune cells in abdominal aortic tissue from the AAA model after 1 week of AngII infusion. ( B ) Representative flow cytometry plots of digested abdominal aortic tissue 1 week after AngII infusion ( n = 5 mice per group). ( C ) Quantification of immune cell populations in digested abdominal aortic tissue (absolute number per 100 mg). Neut, neutrophils; Ly6C hi Mono, Ly6C hi monocytes; Mac, macrophages; CCR2 + Mac, CCR2 + macrophages. Saline, n = 6; AngII, n = 5; B cells and T cells, n = 5 per group. * P < 0.05 and ** P < 0.01, by Mann-Whitney U test. ( D ) Representative images of CD68, α–smooth muscle actin (α-SMA), CD31/platelet endothelial cell adhesion molecule 1 (PECAM-1), and DAPI immunofluorescence staining of abdominal aortic tissue from aortas of control and 20% Tet2 -KO BM recipient mice after 1 week of AngII infusion. Images are representative of 8 mice per genotype. Scale bars: 100 μm. ( E ) Schematic of RNA-seq analysis of sorted macrophages from abdominal aortas of 100% Tet2 -KO BM recipient mice after 1 week of AngII infusion. ( F and G ) Upregulated and downregulated genes are presented as a heatmap ( F ) and volcano plot ( G ). ( H ) Gene Ontology enrichment analysis of upregulated genes in Tet2 -deficient macrophages. The table lists the predominant genes ranked by q value among significantly enriched pathways. ( I and J ) In vitro differentiation of BM-derived macrophages. ( I and J ) Representative images and quantification of the TRAP + cell-to-macrophage ratio after RANKL stimulation from 8 independent biological replicates per genotype. *** P < 0.001, by Mann-Whitney U test. ( K <t>)</t> <t>qRT-PCR</t> analysis of BM-derived macrophages 6 hours after stimulation with 10 ng/mL LPS ( n = 6 independent biological replicates per genotype). **** P < 0.0001, by Mann-Whitney U test.
Reverse Transcription Quantitative Pcr Rt Qpcr, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+pcr/ChamQ+SYBR+qPCR+Master+Mix+-+Low+ROX+Premixed/pmc13121626-302-0-15
Average 96 stars, based on 1 article reviews
reverse transcription quantitative pcr rt qpcr - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
quantitive reverse transcription polymerase chain reaction qrt pcr system  (Roche)
99
Roche quantitive reverse transcription polymerase chain reaction qrt pcr system
( A ) Schematic of flow cytometric analysis and quantification of immune cells in abdominal aortic tissue from the AAA model after 1 week of AngII infusion. ( B ) Representative flow cytometry plots of digested abdominal aortic tissue 1 week after AngII infusion ( n = 5 mice per group). ( C ) Quantification of immune cell populations in digested abdominal aortic tissue (absolute number per 100 mg). Neut, neutrophils; Ly6C hi Mono, Ly6C hi monocytes; Mac, macrophages; CCR2 + Mac, CCR2 + macrophages. Saline, n = 6; AngII, n = 5; B cells and T cells, n = 5 per group. * P < 0.05 and ** P < 0.01, by Mann-Whitney U test. ( D ) Representative images of CD68, α–smooth muscle actin (α-SMA), CD31/platelet endothelial cell adhesion molecule 1 (PECAM-1), and DAPI immunofluorescence staining of abdominal aortic tissue from aortas of control and 20% Tet2 -KO BM recipient mice after 1 week of AngII infusion. Images are representative of 8 mice per genotype. Scale bars: 100 μm. ( E ) Schematic of RNA-seq analysis of sorted macrophages from abdominal aortas of 100% Tet2 -KO BM recipient mice after 1 week of AngII infusion. ( F and G ) Upregulated and downregulated genes are presented as a heatmap ( F ) and volcano plot ( G ). ( H ) Gene Ontology enrichment analysis of upregulated genes in Tet2 -deficient macrophages. The table lists the predominant genes ranked by q value among significantly enriched pathways. ( I and J ) In vitro differentiation of BM-derived macrophages. ( I and J ) Representative images and quantification of the TRAP + cell-to-macrophage ratio after RANKL stimulation from 8 independent biological replicates per genotype. *** P < 0.001, by Mann-Whitney U test. ( K <t>)</t> <t>qRT-PCR</t> analysis of BM-derived macrophages 6 hours after stimulation with 10 ng/mL LPS ( n = 6 independent biological replicates per genotype). **** P < 0.0001, by Mann-Whitney U test.
Quantitive Reverse Transcription Polymerase Chain Reaction Qrt Pcr System, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quantitative+reverse+transcription+pcr/LightCycler+480+System/pmc12853962-200-12-19
Average 99 stars, based on 1 article reviews
quantitive reverse transcription polymerase chain reaction qrt pcr system - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

Image Search Results


( A ) Schematic of flow cytometric analysis and quantification of immune cells in abdominal aortic tissue from the AAA model after 1 week of AngII infusion. ( B ) Representative flow cytometry plots of digested abdominal aortic tissue 1 week after AngII infusion ( n = 5 mice per group). ( C ) Quantification of immune cell populations in digested abdominal aortic tissue (absolute number per 100 mg). Neut, neutrophils; Ly6C hi Mono, Ly6C hi monocytes; Mac, macrophages; CCR2 + Mac, CCR2 + macrophages. Saline, n = 6; AngII, n = 5; B cells and T cells, n = 5 per group. * P < 0.05 and ** P < 0.01, by Mann-Whitney U test. ( D ) Representative images of CD68, α–smooth muscle actin (α-SMA), CD31/platelet endothelial cell adhesion molecule 1 (PECAM-1), and DAPI immunofluorescence staining of abdominal aortic tissue from aortas of control and 20% Tet2 -KO BM recipient mice after 1 week of AngII infusion. Images are representative of 8 mice per genotype. Scale bars: 100 μm. ( E ) Schematic of RNA-seq analysis of sorted macrophages from abdominal aortas of 100% Tet2 -KO BM recipient mice after 1 week of AngII infusion. ( F and G ) Upregulated and downregulated genes are presented as a heatmap ( F ) and volcano plot ( G ). ( H ) Gene Ontology enrichment analysis of upregulated genes in Tet2 -deficient macrophages. The table lists the predominant genes ranked by q value among significantly enriched pathways. ( I and J ) In vitro differentiation of BM-derived macrophages. ( I and J ) Representative images and quantification of the TRAP + cell-to-macrophage ratio after RANKL stimulation from 8 independent biological replicates per genotype. *** P < 0.001, by Mann-Whitney U test. ( K ) qRT-PCR analysis of BM-derived macrophages 6 hours after stimulation with 10 ng/mL LPS ( n = 6 independent biological replicates per genotype). **** P < 0.0001, by Mann-Whitney U test.

Journal: The Journal of Clinical Investigation

Article Title: Tet2-driven clonal hematopoiesis drives aortic aneurysm via macrophage-to-osteoclast–like differentiation

doi: 10.1172/JCI198708

Figure Lengend Snippet: ( A ) Schematic of flow cytometric analysis and quantification of immune cells in abdominal aortic tissue from the AAA model after 1 week of AngII infusion. ( B ) Representative flow cytometry plots of digested abdominal aortic tissue 1 week after AngII infusion ( n = 5 mice per group). ( C ) Quantification of immune cell populations in digested abdominal aortic tissue (absolute number per 100 mg). Neut, neutrophils; Ly6C hi Mono, Ly6C hi monocytes; Mac, macrophages; CCR2 + Mac, CCR2 + macrophages. Saline, n = 6; AngII, n = 5; B cells and T cells, n = 5 per group. * P < 0.05 and ** P < 0.01, by Mann-Whitney U test. ( D ) Representative images of CD68, α–smooth muscle actin (α-SMA), CD31/platelet endothelial cell adhesion molecule 1 (PECAM-1), and DAPI immunofluorescence staining of abdominal aortic tissue from aortas of control and 20% Tet2 -KO BM recipient mice after 1 week of AngII infusion. Images are representative of 8 mice per genotype. Scale bars: 100 μm. ( E ) Schematic of RNA-seq analysis of sorted macrophages from abdominal aortas of 100% Tet2 -KO BM recipient mice after 1 week of AngII infusion. ( F and G ) Upregulated and downregulated genes are presented as a heatmap ( F ) and volcano plot ( G ). ( H ) Gene Ontology enrichment analysis of upregulated genes in Tet2 -deficient macrophages. The table lists the predominant genes ranked by q value among significantly enriched pathways. ( I and J ) In vitro differentiation of BM-derived macrophages. ( I and J ) Representative images and quantification of the TRAP + cell-to-macrophage ratio after RANKL stimulation from 8 independent biological replicates per genotype. *** P < 0.001, by Mann-Whitney U test. ( K ) qRT-PCR analysis of BM-derived macrophages 6 hours after stimulation with 10 ng/mL LPS ( n = 6 independent biological replicates per genotype). **** P < 0.0001, by Mann-Whitney U test.

Article Snippet: Lentiviral titers were determined using a Lenti-X quantitative reverse transcription PCR (qRT-PCR) Titration Kit (catalog 631235, Clontech).

Techniques: Flow Cytometry, Saline, MANN-WHITNEY, Immunofluorescence, Staining, Control, RNA Sequencing, In Vitro, Derivative Assay, Quantitative RT-PCR